Biolytic Lab Performance, Inc.

Our Manual Deprotection Clamp is designed to provide a positive seal to all 96 wells of a 96 well plate. The seal is maintained under pressure and under temperatures up to 70 degrees C. This device is designed to allow for easy deprotection (removal of side chain protecting groups) of synthesized oligos. Cleaved oligos should have been deposited in a deep well plate or in a rack of tubes depending on what the user prefers. A seal is applied to the top of the Plate or Rack of tubes to seal the tops so the Cleavage Reagent cannot escape when the device is heated. The Plate or Rack of tubes with the top seal is placed in the Pneumatic Plate Clamp. Air or Gas pressure is applied to the Clamp to force the top seal in place. The assembly is placed in a heating device such as an Oven or a Water Bath. In order to maintain a vapor tight seal it is recommended that the Heat source is not set to a temperature above 75 degrees C. Allow the Clamped Plate / Rack assembly to stay in the Oven or Water bath as long as necessary to completely deprotect the oligo side chains. Typically this would be about 3 to 4 hours at 75 Degrees C. If you use dG IBU, deprotection needs to be longer. Also available in an automatic sealing configuration. Custom configurations are available. Request a quote for more information. Specification Plate format 96 and 384 Automatic Seal No Key Feature Benefit Oven and water safe The whole clamp can be placed in an oven or water bath for easy operation.